CD163 antibody

Catalog No. ABIN5541127

This anti-CD163 antibody is a Mouse Monoclonal antibody detecting CD163 in WB, FACS, IP, IHC (fro) and Func. Suitable for Pig.

Quick Overview for CD163 antibody (ABIN5541127)

Target: CD163

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 2A10-11

Product Details anti-CD163 Antibody

Specificity: This antibody recognizes Porcine CD163, a 120kD cell surface glycoprotein that is expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. The antibody detects a band of approximately 120kD in alveolar macrophage cell lysates.

Purification: Affinity Chromatography on Protein A

Immunogen: Porcine alveolar macrophages

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections. Western Blotting (Non-reducing conditions). Clone 2A10/11 detects a band of approximately 120kD in alveolar macrophage cell lysates under non-reducing conditions. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antiboy to label 1x10e6 cells in 100 μL. Functional Assays: Clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (See Sanchez-Torres et al. 2003). Removal of Sodium Azide is recommended prior to use.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS Preservatives: 0.09 % Sodium Azide Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD163

Target: CD163

Alternative Name: cd163

Background: CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.

UniProt: Q2VL90